Journal: Bioactive Materials
Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer
doi: 10.1016/j.bioactmat.2026.02.046
Figure Lengend Snippet: Pure Mg, Mg–Cu, and Mg–Cu–Zn alloys inhibit tumor cell proliferation in immunocompetent C57BL/6 mice. (a) Representative H&E-stained tumor images showing necrosis and Ki-67 IHC staining in serial sections. Red dashed boxes indicate necrotic areas. (b) Quantification of tumor necrosis rates across groups. (c) Comparison of Ki-67 labeling index between perinecrotic and non-perinecrotic tumor cells. (d, e) Statistical analysis of Ki-67 labeling index in perinecrotic and non-perinecrotic regions across groups. (f) Representative IHC images of Ki-67 expression in viable tumor cells from perinecrotic and non-perinecrotic regions. Scale bar: 200 μm. p < 0.05 (∗), p < 0.001 (∗∗∗).
Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).
Techniques: Staining, Immunohistochemistry, Comparison, Labeling, Expressing